GlutaredoxinV1, Main, Exploration, bibRecord, 000307

Systematic in vitro assessment of responses of roGFP2-based probes to physiologically relevant oxidant species.

Identifieur interne : 000307 ( Main/Exploration ); précédent : 000306; suivant : 000308

Systematic in vitro assessment of responses of roGFP2-based probes to physiologically relevant oxidant species.

Auteurs : Alexandra Müller [Allemagne] ; Jannis F. Schneider [Allemagne] ; Adriana Degrossoli [Allemagne] ; Nataliya Lupilova [Allemagne] ; Tobias P. Dick [Allemagne] ; Lars I. Leichert [Allemagne]

Source :

Free radical biology & medicine [ 1873-4596 ] ; 2017.

RBID : pubmed:28242229

Descripteurs français

English descriptors

KwdEn :
- Glutaredoxins (chemistry), Glutathione (chemistry), Glutathione (metabolism), Glutathione Disulfide (chemistry), Glutathione Disulfide (isolation & purification), Green Fluorescent Proteins (chemistry), Green Fluorescent Proteins (genetics), Hydrogen Peroxide (chemistry), Hydrogen Peroxide (isolation & purification), Nitric Oxide (chemistry), Nitric Oxide (metabolism), Oxidants (chemistry), Oxidants (metabolism), Oxidation-Reduction (MeSH), Peroxynitrous Acid (chemistry), Peroxynitrous Acid (metabolism), Phagocytes (metabolism), Reactive Nitrogen Species (metabolism), Reactive Oxygen Species (metabolism).
MESH :
- chemical , chemistry : Glutaredoxins, Glutathione, Glutathione Disulfide, Green Fluorescent Proteins, Hydrogen Peroxide, Nitric Oxide, Oxidants, Peroxynitrous Acid.
- chemical , genetics : Green Fluorescent Proteins.
- chemical , isolation & purification : Glutathione Disulfide, Hydrogen Peroxide.
- chemical , metabolism : Glutathione, Nitric Oxide, Oxidants, Peroxynitrous Acid, Reactive Nitrogen Species, Reactive Oxygen Species.
- metabolism : Phagocytes.
- Oxidation-Reduction.

Abstract

The genetically encoded probes roGFP2-Orp1 and Grx1-roGFP2 have been designed to be selectively oxidized by hydrogen peroxide (H₂O₂) and glutathione disulfide (GSSG), respectively. Both probes have demonstrated such selectivity in a broad variety of systems and conditions. In this study, we systematically compared the in vitro response of roGFP2, roGFP2-Orp1 and Grx1-roGFP2 to increasing amounts of various oxidant species that may also occur in biological settings. We conclude that the previously established oxidant selectivity is highly robust and likely to be maintained under most physiological conditions. Yet, we also find that hypochlorous acid, known to be produced in the phagocyte respiratory burst, can lead to non-selective oxidation of roGFP2-based probes at concentrations ≥2µM, in vitro. Further, we confirm that polysulfides trigger direct roGFP2 responses. A side-by-side comparison of all three probes can be used to reveal micromolar amounts of hypochlorous acid or polysulfides.

DOI: 10.1016/j.freeradbiomed.2017.02.044
PubMed: 28242229

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000365
to stream Main, to step Curation: 000365

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Systematic in vitro assessment of responses of roGFP2-based probes to physiologically relevant oxidant species.</title>
<author><name sortKey="Muller, Alexandra" sort="Muller, Alexandra" uniqKey="Muller A" first="Alexandra" last="Müller">Alexandra Müller</name>
<affiliation wicri:level="1"><nlm:affiliation>Institute of Biochemistry and Pathobiochemistry - Microbial Biochemistry, Ruhr-University Bochum, 44780 Bochum, Germany. Electronic address: alexandra.mueller-2@ruhr-uni-bochum.de.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Institute of Biochemistry and Pathobiochemistry - Microbial Biochemistry, Ruhr-University Bochum, 44780 Bochum</wicri:regionArea>
<wicri:noRegion>44780 Bochum</wicri:noRegion>
<wicri:noRegion>44780 Bochum</wicri:noRegion>
<wicri:noRegion>44780 Bochum</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Schneider, Jannis F" sort="Schneider, Jannis F" uniqKey="Schneider J" first="Jannis F" last="Schneider">Jannis F. Schneider</name>
<affiliation wicri:level="1"><nlm:affiliation>Institute of Biochemistry and Pathobiochemistry - Microbial Biochemistry, Ruhr-University Bochum, 44780 Bochum, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Institute of Biochemistry and Pathobiochemistry - Microbial Biochemistry, Ruhr-University Bochum, 44780 Bochum</wicri:regionArea>
<wicri:noRegion>44780 Bochum</wicri:noRegion>
<wicri:noRegion>44780 Bochum</wicri:noRegion>
<wicri:noRegion>44780 Bochum</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Degrossoli, Adriana" sort="Degrossoli, Adriana" uniqKey="Degrossoli A" first="Adriana" last="Degrossoli">Adriana Degrossoli</name>
<affiliation wicri:level="1"><nlm:affiliation>Institute of Biochemistry and Pathobiochemistry - Microbial Biochemistry, Ruhr-University Bochum, 44780 Bochum, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Institute of Biochemistry and Pathobiochemistry - Microbial Biochemistry, Ruhr-University Bochum, 44780 Bochum</wicri:regionArea>
<wicri:noRegion>44780 Bochum</wicri:noRegion>
<wicri:noRegion>44780 Bochum</wicri:noRegion>
<wicri:noRegion>44780 Bochum</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lupilova, Nataliya" sort="Lupilova, Nataliya" uniqKey="Lupilova N" first="Nataliya" last="Lupilova">Nataliya Lupilova</name>
<affiliation wicri:level="1"><nlm:affiliation>Institute of Biochemistry and Pathobiochemistry - Microbial Biochemistry, Ruhr-University Bochum, 44780 Bochum, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Institute of Biochemistry and Pathobiochemistry - Microbial Biochemistry, Ruhr-University Bochum, 44780 Bochum</wicri:regionArea>
<wicri:noRegion>44780 Bochum</wicri:noRegion>
<wicri:noRegion>44780 Bochum</wicri:noRegion>
<wicri:noRegion>44780 Bochum</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Dick, Tobias P" sort="Dick, Tobias P" uniqKey="Dick T" first="Tobias P" last="Dick">Tobias P. Dick</name>
<affiliation wicri:level="3"><nlm:affiliation>Division of Redox Regulation, DKFZ-ZMBH Alliance, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 280, 69120 Heidelberg, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Division of Redox Regulation, DKFZ-ZMBH Alliance, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 280, 69120 Heidelberg</wicri:regionArea>
<placeName><region type="land" nuts="1">Bade-Wurtemberg</region>
<region type="district" nuts="2">District de Karlsruhe</region>
<settlement type="city">Heidelberg</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Leichert, Lars I" sort="Leichert, Lars I" uniqKey="Leichert L" first="Lars I" last="Leichert">Lars I. Leichert</name>
<affiliation wicri:level="1"><nlm:affiliation>Institute of Biochemistry and Pathobiochemistry - Microbial Biochemistry, Ruhr-University Bochum, 44780 Bochum, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Institute of Biochemistry and Pathobiochemistry - Microbial Biochemistry, Ruhr-University Bochum, 44780 Bochum</wicri:regionArea>
<wicri:noRegion>44780 Bochum</wicri:noRegion>
<wicri:noRegion>44780 Bochum</wicri:noRegion>
<wicri:noRegion>44780 Bochum</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2017">2017</date>
<idno type="RBID">pubmed:28242229</idno>
<idno type="pmid">28242229</idno>
<idno type="doi">10.1016/j.freeradbiomed.2017.02.044</idno>
<idno type="wicri:Area/Main/Corpus">000365</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000365</idno>
<idno type="wicri:Area/Main/Curation">000365</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000365</idno>
<idno type="wicri:Area/Main/Exploration">000365</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Systematic in vitro assessment of responses of roGFP2-based probes to physiologically relevant oxidant species.</title>
<author><name sortKey="Muller, Alexandra" sort="Muller, Alexandra" uniqKey="Muller A" first="Alexandra" last="Müller">Alexandra Müller</name>
<affiliation wicri:level="1"><nlm:affiliation>Institute of Biochemistry and Pathobiochemistry - Microbial Biochemistry, Ruhr-University Bochum, 44780 Bochum, Germany. Electronic address: alexandra.mueller-2@ruhr-uni-bochum.de.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Institute of Biochemistry and Pathobiochemistry - Microbial Biochemistry, Ruhr-University Bochum, 44780 Bochum</wicri:regionArea>
<wicri:noRegion>44780 Bochum</wicri:noRegion>
<wicri:noRegion>44780 Bochum</wicri:noRegion>
<wicri:noRegion>44780 Bochum</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Schneider, Jannis F" sort="Schneider, Jannis F" uniqKey="Schneider J" first="Jannis F" last="Schneider">Jannis F. Schneider</name>
<affiliation wicri:level="1"><nlm:affiliation>Institute of Biochemistry and Pathobiochemistry - Microbial Biochemistry, Ruhr-University Bochum, 44780 Bochum, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Institute of Biochemistry and Pathobiochemistry - Microbial Biochemistry, Ruhr-University Bochum, 44780 Bochum</wicri:regionArea>
<wicri:noRegion>44780 Bochum</wicri:noRegion>
<wicri:noRegion>44780 Bochum</wicri:noRegion>
<wicri:noRegion>44780 Bochum</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Degrossoli, Adriana" sort="Degrossoli, Adriana" uniqKey="Degrossoli A" first="Adriana" last="Degrossoli">Adriana Degrossoli</name>
<affiliation wicri:level="1"><nlm:affiliation>Institute of Biochemistry and Pathobiochemistry - Microbial Biochemistry, Ruhr-University Bochum, 44780 Bochum, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Institute of Biochemistry and Pathobiochemistry - Microbial Biochemistry, Ruhr-University Bochum, 44780 Bochum</wicri:regionArea>
<wicri:noRegion>44780 Bochum</wicri:noRegion>
<wicri:noRegion>44780 Bochum</wicri:noRegion>
<wicri:noRegion>44780 Bochum</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lupilova, Nataliya" sort="Lupilova, Nataliya" uniqKey="Lupilova N" first="Nataliya" last="Lupilova">Nataliya Lupilova</name>
<affiliation wicri:level="1"><nlm:affiliation>Institute of Biochemistry and Pathobiochemistry - Microbial Biochemistry, Ruhr-University Bochum, 44780 Bochum, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Institute of Biochemistry and Pathobiochemistry - Microbial Biochemistry, Ruhr-University Bochum, 44780 Bochum</wicri:regionArea>
<wicri:noRegion>44780 Bochum</wicri:noRegion>
<wicri:noRegion>44780 Bochum</wicri:noRegion>
<wicri:noRegion>44780 Bochum</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Dick, Tobias P" sort="Dick, Tobias P" uniqKey="Dick T" first="Tobias P" last="Dick">Tobias P. Dick</name>
<affiliation wicri:level="3"><nlm:affiliation>Division of Redox Regulation, DKFZ-ZMBH Alliance, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 280, 69120 Heidelberg, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Division of Redox Regulation, DKFZ-ZMBH Alliance, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 280, 69120 Heidelberg</wicri:regionArea>
<placeName><region type="land" nuts="1">Bade-Wurtemberg</region>
<region type="district" nuts="2">District de Karlsruhe</region>
<settlement type="city">Heidelberg</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Leichert, Lars I" sort="Leichert, Lars I" uniqKey="Leichert L" first="Lars I" last="Leichert">Lars I. Leichert</name>
<affiliation wicri:level="1"><nlm:affiliation>Institute of Biochemistry and Pathobiochemistry - Microbial Biochemistry, Ruhr-University Bochum, 44780 Bochum, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Institute of Biochemistry and Pathobiochemistry - Microbial Biochemistry, Ruhr-University Bochum, 44780 Bochum</wicri:regionArea>
<wicri:noRegion>44780 Bochum</wicri:noRegion>
<wicri:noRegion>44780 Bochum</wicri:noRegion>
<wicri:noRegion>44780 Bochum</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">Free radical biology & medicine</title>
<idno type="eISSN">1873-4596</idno>
<imprint><date when="2017" type="published">2017</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Glutaredoxins (chemistry)</term>
<term>Glutathione (chemistry)</term>
<term>Glutathione (metabolism)</term>
<term>Glutathione Disulfide (chemistry)</term>
<term>Glutathione Disulfide (isolation & purification)</term>
<term>Green Fluorescent Proteins (chemistry)</term>
<term>Green Fluorescent Proteins (genetics)</term>
<term>Hydrogen Peroxide (chemistry)</term>
<term>Hydrogen Peroxide (isolation & purification)</term>
<term>Nitric Oxide (chemistry)</term>
<term>Nitric Oxide (metabolism)</term>
<term>Oxidants (chemistry)</term>
<term>Oxidants (metabolism)</term>
<term>Oxidation-Reduction (MeSH)</term>
<term>Peroxynitrous Acid (chemistry)</term>
<term>Peroxynitrous Acid (metabolism)</term>
<term>Phagocytes (metabolism)</term>
<term>Reactive Nitrogen Species (metabolism)</term>
<term>Reactive Oxygen Species (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Acide peroxynitreux (composition chimique)</term>
<term>Acide peroxynitreux (métabolisme)</term>
<term>Disulfure de glutathion (composition chimique)</term>
<term>Disulfure de glutathion (isolement et purification)</term>
<term>Espèces réactives de l'azote (métabolisme)</term>
<term>Espèces réactives de l'oxygène (métabolisme)</term>
<term>Glutarédoxines (composition chimique)</term>
<term>Glutathion (composition chimique)</term>
<term>Glutathion (métabolisme)</term>
<term>Monoxyde d'azote (composition chimique)</term>
<term>Monoxyde d'azote (métabolisme)</term>
<term>Oxydants (composition chimique)</term>
<term>Oxydants (métabolisme)</term>
<term>Oxydoréduction (MeSH)</term>
<term>Peroxyde d'hydrogène (composition chimique)</term>
<term>Peroxyde d'hydrogène (isolement et purification)</term>
<term>Phagocytes (métabolisme)</term>
<term>Protéines à fluorescence verte (composition chimique)</term>
<term>Protéines à fluorescence verte (génétique)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Glutaredoxins</term>
<term>Glutathione</term>
<term>Glutathione Disulfide</term>
<term>Green Fluorescent Proteins</term>
<term>Hydrogen Peroxide</term>
<term>Nitric Oxide</term>
<term>Oxidants</term>
<term>Peroxynitrous Acid</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Green Fluorescent Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Glutathione Disulfide</term>
<term>Hydrogen Peroxide</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Glutathione</term>
<term>Nitric Oxide</term>
<term>Oxidants</term>
<term>Peroxynitrous Acid</term>
<term>Reactive Nitrogen Species</term>
<term>Reactive Oxygen Species</term>
</keywords>
<keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Acide peroxynitreux</term>
<term>Disulfure de glutathion</term>
<term>Glutarédoxines</term>
<term>Glutathion</term>
<term>Monoxyde d'azote</term>
<term>Oxydants</term>
<term>Peroxyde d'hydrogène</term>
<term>Protéines à fluorescence verte</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines à fluorescence verte</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Disulfure de glutathion</term>
<term>Peroxyde d'hydrogène</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Phagocytes</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Acide peroxynitreux</term>
<term>Espèces réactives de l'azote</term>
<term>Espèces réactives de l'oxygène</term>
<term>Glutathion</term>
<term>Monoxyde d'azote</term>
<term>Oxydants</term>
<term>Phagocytes</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Oxidation-Reduction</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Oxydoréduction</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The genetically encoded probes roGFP2-Orp1 and Grx1-roGFP2 have been designed to be selectively oxidized by hydrogen peroxide (H<sub>2</sub>
O<sub>2</sub>
) and glutathione disulfide (GSSG), respectively. Both probes have demonstrated such selectivity in a broad variety of systems and conditions. In this study, we systematically compared the in vitro response of roGFP2, roGFP2-Orp1 and Grx1-roGFP2 to increasing amounts of various oxidant species that may also occur in biological settings. We conclude that the previously established oxidant selectivity is highly robust and likely to be maintained under most physiological conditions. Yet, we also find that hypochlorous acid, known to be produced in the phagocyte respiratory burst, can lead to non-selective oxidation of roGFP2-based probes at concentrations ≥2µM, in vitro. Further, we confirm that polysulfides trigger direct roGFP2 responses. A side-by-side comparison of all three probes can be used to reveal micromolar amounts of hypochlorous acid or polysulfides.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28242229</PMID>
<DateCompleted><Year>2017</Year>
<Month>12</Month>
<Day>15</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>03</Month>
<Day>15</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-4596</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>106</Volume>
<PubDate><Year>2017</Year>
<Month>05</Month>
</PubDate>
</JournalIssue>
<Title>Free radical biology & medicine</Title>
<ISOAbbreviation>Free Radic Biol Med</ISOAbbreviation>
</Journal>
<ArticleTitle>Systematic in vitro assessment of responses of roGFP2-based probes to physiologically relevant oxidant species.</ArticleTitle>
<Pagination><MedlinePgn>329-338</MedlinePgn>
</Pagination>
<ELocationID EIdType="pii" ValidYN="Y">S0891-5849(17)30116-8</ELocationID>
<ELocationID EIdType="doi" ValidYN="Y">10.1016/j.freeradbiomed.2017.02.044</ELocationID>
<Abstract><AbstractText>The genetically encoded probes roGFP2-Orp1 and Grx1-roGFP2 have been designed to be selectively oxidized by hydrogen peroxide (H<sub>2</sub>
O<sub>2</sub>
) and glutathione disulfide (GSSG), respectively. Both probes have demonstrated such selectivity in a broad variety of systems and conditions. In this study, we systematically compared the in vitro response of roGFP2, roGFP2-Orp1 and Grx1-roGFP2 to increasing amounts of various oxidant species that may also occur in biological settings. We conclude that the previously established oxidant selectivity is highly robust and likely to be maintained under most physiological conditions. Yet, we also find that hypochlorous acid, known to be produced in the phagocyte respiratory burst, can lead to non-selective oxidation of roGFP2-based probes at concentrations ≥2µM, in vitro. Further, we confirm that polysulfides trigger direct roGFP2 responses. A side-by-side comparison of all three probes can be used to reveal micromolar amounts of hypochlorous acid or polysulfides.</AbstractText>
<CopyrightInformation>Copyright © 2017 Elsevier Inc. All rights reserved.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Müller</LastName>
<ForeName>Alexandra</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Institute of Biochemistry and Pathobiochemistry - Microbial Biochemistry, Ruhr-University Bochum, 44780 Bochum, Germany. Electronic address: alexandra.mueller-2@ruhr-uni-bochum.de.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Schneider</LastName>
<ForeName>Jannis F</ForeName>
<Initials>JF</Initials>
<AffiliationInfo><Affiliation>Institute of Biochemistry and Pathobiochemistry - Microbial Biochemistry, Ruhr-University Bochum, 44780 Bochum, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Degrossoli</LastName>
<ForeName>Adriana</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Institute of Biochemistry and Pathobiochemistry - Microbial Biochemistry, Ruhr-University Bochum, 44780 Bochum, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Lupilova</LastName>
<ForeName>Nataliya</ForeName>
<Initials>N</Initials>
<AffiliationInfo><Affiliation>Institute of Biochemistry and Pathobiochemistry - Microbial Biochemistry, Ruhr-University Bochum, 44780 Bochum, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Dick</LastName>
<ForeName>Tobias P</ForeName>
<Initials>TP</Initials>
<AffiliationInfo><Affiliation>Division of Redox Regulation, DKFZ-ZMBH Alliance, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 280, 69120 Heidelberg, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Leichert</LastName>
<ForeName>Lars I</ForeName>
<Initials>LI</Initials>
<AffiliationInfo><Affiliation>Institute of Biochemistry and Pathobiochemistry - Microbial Biochemistry, Ruhr-University Bochum, 44780 Bochum, Germany.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2017</Year>
<Month>02</Month>
<Day>27</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>Free Radic Biol Med</MedlineTA>
<NlmUniqueID>8709159</NlmUniqueID>
<ISSNLinking>0891-5849</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C516008">GLRX2 protein, human</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D054477">Glutaredoxins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D016877">Oxidants</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D026361">Reactive Nitrogen Species</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D017382">Reactive Oxygen Species</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>14691-52-2</RegistryNumber>
<NameOfSubstance UI="D030421">Peroxynitrous Acid</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>147336-22-9</RegistryNumber>
<NameOfSubstance UI="D049452">Green Fluorescent Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>31C4KY9ESH</RegistryNumber>
<NameOfSubstance UI="D009569">Nitric Oxide</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>BBX060AN9V</RegistryNumber>
<NameOfSubstance UI="D006861">Hydrogen Peroxide</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>GAN16C9B8O</RegistryNumber>
<NameOfSubstance UI="D005978">Glutathione</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>ULW86O013H</RegistryNumber>
<NameOfSubstance UI="D019803">Glutathione Disulfide</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D054477" MajorTopicYN="N">Glutaredoxins</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005978" MajorTopicYN="N">Glutathione</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019803" MajorTopicYN="N">Glutathione Disulfide</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
<QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D049452" MajorTopicYN="N">Green Fluorescent Proteins</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006861" MajorTopicYN="N">Hydrogen Peroxide</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009569" MajorTopicYN="N">Nitric Oxide</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016877" MajorTopicYN="N">Oxidants</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010084" MajorTopicYN="N">Oxidation-Reduction</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D030421" MajorTopicYN="N">Peroxynitrous Acid</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010586" MajorTopicYN="N">Phagocytes</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D026361" MajorTopicYN="N">Reactive Nitrogen Species</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017382" MajorTopicYN="N">Reactive Oxygen Species</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Genetically encoded redox probes</Keyword>
<Keyword MajorTopicYN="Y">Glutathione</Keyword>
<Keyword MajorTopicYN="Y">H(2)O(2)</Keyword>
<Keyword MajorTopicYN="Y">HOCl</Keyword>
<Keyword MajorTopicYN="Y">Nitric oxide</Keyword>
<Keyword MajorTopicYN="Y">Peroxynitrite</Keyword>
<Keyword MajorTopicYN="Y">Polysulfides</Keyword>
</KeywordList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year>
<Month>11</Month>
<Day>03</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="revised"><Year>2017</Year>
<Month>02</Month>
<Day>03</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2017</Year>
<Month>02</Month>
<Day>22</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2017</Year>
<Month>3</Month>
<Day>1</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2017</Year>
<Month>12</Month>
<Day>16</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2017</Year>
<Month>3</Month>
<Day>1</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">28242229</ArticleId>
<ArticleId IdType="pii">S0891-5849(17)30116-8</ArticleId>
<ArticleId IdType="doi">10.1016/j.freeradbiomed.2017.02.044</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Allemagne</li>
</country>
<region><li>Bade-Wurtemberg</li>
<li>District de Karlsruhe</li>
</region>
<settlement><li>Heidelberg</li>
</settlement>
</list>
<tree><country name="Allemagne"><noRegion><name sortKey="Muller, Alexandra" sort="Muller, Alexandra" uniqKey="Muller A" first="Alexandra" last="Müller">Alexandra Müller</name>
</noRegion>
<name sortKey="Degrossoli, Adriana" sort="Degrossoli, Adriana" uniqKey="Degrossoli A" first="Adriana" last="Degrossoli">Adriana Degrossoli</name>
<name sortKey="Dick, Tobias P" sort="Dick, Tobias P" uniqKey="Dick T" first="Tobias P" last="Dick">Tobias P. Dick</name>
<name sortKey="Leichert, Lars I" sort="Leichert, Lars I" uniqKey="Leichert L" first="Lars I" last="Leichert">Lars I. Leichert</name>
<name sortKey="Lupilova, Nataliya" sort="Lupilova, Nataliya" uniqKey="Lupilova N" first="Nataliya" last="Lupilova">Nataliya Lupilova</name>
<name sortKey="Schneider, Jannis F" sort="Schneider, Jannis F" uniqKey="Schneider J" first="Jannis F" last="Schneider">Jannis F. Schneider</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Bois/explor/GlutaredoxinV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000307 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000307 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Bois
   |area=    GlutaredoxinV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:28242229
   |texte=   Systematic in vitro assessment of responses of roGFP2-based probes to physiologically relevant oxidant species.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:28242229" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a GlutaredoxinV1

This area was generated with Dilib version V0.6.37.
Data generation: Wed Nov 18 15:13:42 2020. Site generation: Wed Nov 18 15:16:12 2020

	Serveur d'exploration sur la glutarédoxine
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur la glutarédoxine

Systematic in vitro assessment of responses of roGFP2-based probes to physiologically relevant oxidant species.

Systematic in vitro assessment of responses of roGFP2-based probes to physiologically relevant oxidant species.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki